Cdc42 Protein G12V mutant

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Cdc42 Protein G12V mutant

价格	6800.00
品牌	Bioyears
产地	美国
货号	10109-1
免疫原	Mouse
规格	100μl
技术资料	查看技术资料

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概述

Cdc42 Protein G12V mutant [10109]
[100 µg]

Catalog Number: 10109-1

Product Name: Cdc42 Protein G12V mutant

Synonyms: Cell division cycle 42, G25K, CDC42Hs

Source: Human, recombinant full length, His6-tag

Expression Host: E. col

Molecular Weight: 21 kDa

Purity: >95% by SDS-PAGE

Introduction: Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

Amino Acid Sequence (1-191, G12V)

MQTIKCVVVGDVAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRL
RPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQ
KPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL

Properties

Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.

Physical Appearance (form): White or clear

Concentration: 1 mg/mL

Storage: -80°C

Preparation Instructions:
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Cdc42 G12V was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.

References:
1. Garrett, W. S. . et al., Cell 102: 325-334, 2000.
2. Irie, F. et al., Nature Neurosci. 5: 1117-1118, 2002.
3. Kawasaki, Y. et al., Oncogene 26: 7620-7627, 2007.
4. Manser, E. et al., Nature 363: 364-367, 1993.
5. Musch, A. et al., EMBO J. 20: 2171-2179, 2001.
6. Nalbant, P. et al., Science 305: 1615-1619, 2004.
7. Shen, Y. et al., Dev. Cell 14: 342-353, 2008.
8. Wu, W. J. et al., Nature 405: 800-804, 2000.
9. Wu, X. et al., Genes Dev. 20: 571-585, 2006.
10. Zheng, Y. et al., J. Biol. Chem. 271: 33169-33172, 1996.

Publications:

1. Vibrio parahaemolyticus Effector Proteins Suppress Inflammasome Activation by Interfering with Host Autophagy Signaling
PLoS Pathog. 2013 Jan;9(1):e1003142

2. Interferon gamma induces actin polymerization, Rac1 activation and down regulates phagocytosis in human monocytic cells
Cytokine. 2012 Jan;57(1):158-68

3. Invadopodia and rolling-type motility are specific features of highly invasive p190bcr-abl leukemic cells
European Journal of Cell Biology Volume 91, Issues 11–12, November–December 2012, Pages 978–987

4. Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex
J Cell Biol. 2012 Aug 20;198(4):677-93

5. Polarized migration of lymphatic endothelial cells is critically dependent on podoplanin regulation of Cdc42
Am J Physiol Lung Cell Mol Physiol. 2011 Jan;300(1):L32-42

6. The Aarskog-Scott Syndrome Protein Fgd1 Regulates Podosome Formation and Extracellular Matrix Remodeling in Transforming Growth Factor beta-Stimulated Aortic Endothelial Cells
Mol Cell Biol. 2011 Nov;31(22):4430-41

7. MMP-9 and CXCL8/IL-8 Are Potential Therapeutic Targets in Epidermolysis Bullosa Simplex
PLoS One. 2013 Jul 19;8(7):e70123

8. p21-Activated Kinase (PAK) Regulates Cytoskeletal Reorganization and Directional Migration in Human Neutrophils
PLoS One. 2013 Sep 3;8(9):e73063

9. The Cdc42 Guanine Nucleotide Exchange Factor FGD6 Coordinates Cell Polarity and Endosomal Membrane Recycling in Osteoclasts
J Biol Chem. 2014 Jun 27;289(26):18347-59

10. Cdc42 Inhibitor and Uses Thereof
United States Patent Application 20140194451

11. The α4 Nicotinic Receptor Promotes CD4+ T-Cell Proliferation and a Helper T-Cell Immune Response
Molecular Pharmacology January 2014 vol. 85 no. 1 50-61

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