RhoA Protein

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RhoA Protein

价格	2800.00/4800.00
品牌	Bioyears
产地	美国
货号	10104
免疫原	E. coli
规格	100μl/200μl
技术资料	查看技术资料

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概述

RhoA Protein [10104]
[100 µg]

Catalog Number: 10104

Product Name: RhoA Protein

Synonyms: Ras homolog gene family, member A, ARHA, ARH12, RHO12, RHOH12

Source: Human, recombinant full length, His6-tag

Expression Host: E. coli

Molecular Weight: 22 kDa

Purity: >96% by SDS-PAGE

Introduction: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca2+ sensitivity of smooth muscle contraction.

Amino Acid Sequence (1-193)

MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYD
RLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKM
KQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL

Properties

Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.

Physical Appearance (form): White or clear

Concentration: 1 mg/mL

Storage: -80°C

Preparation Instructions:
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged RhoA was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.

References:
1. Canman, J. C. et al., Science 322: 1543-1546, 2008.
2. Guilluy, C. et al., Nature Med. 16: 183-190, 2010.
3. Machacek, M. et al., Nature 461: 99-103, 2009 4. Nakamura, M. et al., Invest. Ophthal. Vis. Sci. 42: 941-947, 2001.
5. Rao, P. V. et al., Invest. Ophthal. Vis. Sci. 42: 1029-1037, 2001.
6. Valderrama, F. et al., Science 311: 377-381, 2006.
7. Wang, H.-R. et al., Science 302: 1775-1779, 2003.
8. Wu, K. Y. et al., Nature 436: 1020-1024, 2005.
9. Yoshida, S.et al., Science 313: 108-111, 2006.

Publications:

1. Ethanol increases p190RhoGAP activity, leading to actin cytoskeleton rearrangements
J Neurochem. 2011 Dec;119(6):1306-16

2. The transcriptional coactivators megakaryoblastic leukemia 1/2 mediate the effects of loss of the tumor suppressor deleted in liver cancer 1
Oncogene. 2012 Aug 30;31(35):3913-23

3. G(alpha)12/13 inhibition enhances the anticancer effect of bortezomib through PSMB5 downregulation
Carcinogenesis. 2010 Jul;31(7):1230-7

4. Transgenesis of the Wolffian duct visualizes dynamic behavior of cells undergoing tubulogenesis in vivo
Development, Growth & Differentiation. Volume 55, Issue 4, pages 579–590, May 2013

5. Foxc2 induces Wnt4 and Bmp4 expression during muscle regeneration and osteogenesis
Cell Death and Differentiation (2013) 20, 1031–1042

6. N-glycosylation induces collagen triple helix repeat containing 1 (CTHRC1) and drives oral cancer cell migration
J Biol Chem. 2013 Jul 12;288(28):20217-27

7. Cyclodextrin protects podocytes in diabetic kidney disease
Diabetes. 2013 Nov;62(11):3817-27

8. Amyloid beta1-42 (Aβ42) up-regulates the expression of sortilin via the p75NTR/RhoA signaling pathway
Journal of Neurochemistry Volume 127, Issue 2, pages 152–162, October 2013

9. Podocyte-specific GLUT4 deficient mice have fewer and larger podocytes and are protected from diabetic nephropathy
Diabetes. 2014 Feb;63(2):701-14

10. Loss of TAK1 increases cell traction force in a ROS-dependent manner to drive epithelial–mesenchymal transition of cancer cells
Cell Death and Disease (2013) 4, e848

11. Syndecan-2 regulation of morphology in breast carcinoma cells is dependent on RhoGTPases
Biochimica et Biophysica Acta (BBA) - General Subjects Volume 1840, Issue 8, August 2014, Pages 2482–2490

12. Epithelial repair is a two-stage process driven first by dying cells and then by their neighbours
J Cell Sci. 2014 Mar 15;127(Pt 6):1229-41

13. MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells
Cytokine Volume 69, Issue 1, September 2014, Pages 47–55

14. Triclosan Potentiates Epithelial-To-Mesenchymal Transition in Anoikis-Resistant Human Lung Cancer Cells
PLoS One. 2014 Oct 16;9(10):e110851

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