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Rab4A Protein Q67L mutant

价格2800.00
品牌Bioyears   
产地美国
货号10131
免疫原E. coli
规格25μl
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  • 概述

    Rab4A Protein Q67L mutant [10131]
    [25 µg]


    Catalog Number:  10131
    Product Name:  Rab4A Protein Q67L mutant
    Synonyms:  Member RAS oncogene family, RAB4, HRES-1/RAB4
    Source:   Human, recombinant full length, His6-tag
    Expression Host:   E. coli
    Molecular Weight:   24 kDa
    Purity:   >95% by SDS-PAGE
    Introduction:   The Rab family of Ras-related GTP-binding protein Rab4 is involved in bidirectional sarcolemmal-vesicular Adrb2 trafficking, which occurs continuously in healthy hearts and is necessary for normal baseline adrenergic responsiveness and resensitization after catecholamine exposure.
    Amino Acid Sequence   (1-213, Q67L)
    MSETYDFLFKFLVIGNAGTGKSCLLHQFIEKKFKDDSNHTIGVEFGSKIINVGGKYVKLQIWDTAGL 
    ERFRSVTRSYYRGAAGALLVYDITSRETYNALTNWLTDARMLASQNIVIILCGNKKDLDADREVTFL 
    EASRFAQENELMFLETSALTGENVEEAFVQCARKILNKIESGELDPERMGSGIQYGDAALRQLRSPRRA 
    QAPNAQECGC
    Properties
    Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
    Physical Appearance (form):  White or clear
    Concentration:  1 mg/mL
    Storage:   -80°C
    Preparation Instructions:  
    Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab4A Q67L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.
    References:  
    1. Barbosa, M. D. F. S. et al., Genomics 30: 439-444, 1995. 
    2. Odley, A. et al., Proc. Nat. Acad. Sci. 101: 7082-7087, 2004. 
    3. Rousseau-Merck, M.-F. et al., Hum. Genet. 86: 350-354, 1991. 
    4. Zahraoui, A. et al., J. Biol. Chem. 264: 12394-12401, 1989.


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