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Rab11 Protein

价格3800.00
品牌Bioyears   
产地美国
货号10126
免疫原E. coli
规格10μl
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  • 概述

    Rab11 Protein [10126]
    [10 µg]


    Catalog Number:  10126
    Product Name:  Rab11 Protein
    Synonyms:  Rab-protein 11, CG5771, DmRab11, Drab11, Rab-r11, RAB11
    Source:   Human, recombinant full length, His6-tag
    Expression Host:    E. coli
    Molecular Weight:   18 kDa
    Purity:   >95% by SDS-PAGE
    Introduction:   Rab11 is a small GTP-binding proteins of the Rab family, such as Rab11A, play essential roles in vesicle and granule targeting. Rab11 is also a GTPase that regulates endosomal trafficking to apical plasma membrane domains in polarized epithelial cells.
    Amino Acid Sequence   (1-173)
    MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDT 
    AGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAV 
    PTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIY
    Properties
    Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
    Physical Appearance (form):  White or clear
    Concentration:  1 mg/mL
    Storage:   -80°C
    Preparation Instructions:  
    Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab11 was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.
    References:  
    1. Bao, X. et al., Europ. J. Biochem. 269: 259-271, 2002. 
    2. Gromov, P. S. et al., FEBS Lett. 429: 359-364, 1998. 
    3. Casanova, J. E. et al., Molec. Biol. Cell 10: 47-61, 1999. 
    4. Drivas, G. T. et al., Oncogene 6: 3-9, 1991. 
    5. Gromov, P. S. et al., FEBS Lett. 429: 359-364, 1998. 
    6. Guichard, A. et al., Nature 467: 854-858, 2010. 
    7. Larance, M. et al., J. Biol. Chem. 280: 37803-37813, 2005. 
    8. Shirane, M. et al., Science 314: 818-821, 2006.



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