武汉天德生物科技有限公司欢迎您！
366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 3800.00 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 10123 |
| 免疫原 | E. coli |
| 规格 | 10μl |
| 技术资料 | 查看技术资料 |
Arf1 Protein Δ17Q71L mutant [10123]
[10 µg]
| Catalog Number: 10123 |
| Product Name: Arf1 Protein Δ17Q71L mutant |
| Synonyms: ADP-ribosylation factor 1 |
| Source: Human recombinant, His6-tag |
| Expression Host: E. coli |
| Molecular Weight: 21 kDa |
| Purity: >95% by SDS-PAGE |
| Introduction: Arf1 is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. |
| Amino Acid Sequence (1-181, Δ17, Q71L) |
| MGNIFANLFKGLFGKK-MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWD VGGLDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAM NAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK |
| Properties |
| Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. |
| Physical Appearance (form): White or clear |
| Concentration: 1 mg/mL |
| Storage: -80°C |
| Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside(DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arf1 Δ17Q71L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining |
 |
| References: 1. Amor, J. C. et al., Nature 372: 704-708, 1994. 2. Bobak, D. A. et al., Proc. Nat. Acad. Sci. 86: 6101-6105, 1989. 3. Hirai, M. et al., Genomics 34: 263-265, 1996. 4. Kumari, S. et al., Cell Biol. 10: 30-41, 2008. 5. Lee, C.-M. et al., J. Biol. Chem. 267: 9028-9034, 1992. 6. Mossessova, E. et al., Cell 92: 415-423, 1998. 7. Peng, Z. G. et al., Biofactors 2: 45-49, 1989. 8. Presley, J. F. et al., Nature 417: 187-193, 2002. 9. Renault, L. et al., Nature 426: 525-530, 2003. |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
