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366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 4800 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 26036 |
| 免疫原 | Mouse |
| 规格 | 100ul |
| 技术资料 | 查看技术资料 |
Ras(G12D) [26036]
[100 µL]
| Catalog Number: 26036 |
| Gene Symbol: RAS(G12D) |
| Description: Anti Ras(G12D) Mouse Monoclonal Antibody |
| Background: Ras(G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G12D, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:100-1:3000 WB: 1:200-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2a |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Ras(G12D) antibody recognizes Ras(G12D) proteins of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 | Western blot analysis of recombinant Ras WT and G12D proteins. Equal amount of purified Ras WT (lane 1, cat. #10172) and G12D (lane 2, cat.# 10154) proteins were loaded and blotted with anti-Ras G12D mouse monoclonal antibody(Cat. # 26036) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat. # 21021） |
| Immunofluorescence: |
|
| Immunofluorescence of cells expressing RAS proteins with anti RAS(G12D) antibody. HEK293T cells were transfected with pCDNA3-GFP Ras(G12D) plasmid, pCDNA3-GFP Ras(WT)plasmid,pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(G12V)plasmid,pCDNA3-GFP Ras(G12C)plasmid, pCDNA3-GFP Ras(G12R)plasmid,or pCDNA3-GFP Ras(G12A)plasmid, then fixed and stained with Anti Ras(G12D) monoclonal antibody (Cat. # 26036). |
| Publications: |
| 1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model Gene Therapy 21, 888-896 (October 2014) |
| 2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136 |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
