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Anti Active Rab3 Mouse Monoclonal Antibody

价格4800.00
品牌Bioyears   
产地美国
货号26920
免疫原Mouse
规格30μl
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  • 概述

    Active Rab3 GTP [26920]
    [30 µL]


    Catalog Number:  26920
    Gene Symbol:   RAB3
    Description:   Anti Active Rab3 Mouse Monoclonal Antibody
    Background:   Rab3 is a member of the Rab-family GTPases and is specifically targeted to synaptic vesicles.
    Immunogen:   Recombinant full length protein of active Rab3A
    Applications:   IP, IHC, IF
    Recommended Dilutions:   
    1 µg for ~2 mg total cellular proteins for IP 
    1:100 dilution for IHC, IF
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Rab3 antibody recognizes active Rab3 proteins of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

    Immunoprecipitation/Western blot:  
    Rab3 activation assay. Purified full-length Rab3A proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti active Rab3 monoclonal antibody (Cat. No. 26920). Immunoblot was with an anti Rab3A polyclonal antibody (Cat. No. 21041).


    Publications:
    1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors
        Hepatology Volume 61, Issue 1, pages 361–374, January 2015
    2.  Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance
        Endocrinology. 2014 Sep;155(9):3315-28
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