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Anti Active Gαo Mouse Monoclonal Antibody

价格4800.00
品牌Bioyears   
产地美国
货号26907
免疫原Mouse
规格30μl
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  • 概述

    Active Gαo GTP [26907]
    [30 µL]


    Catalog Number:  26907
    Gene Symbol:  Gnao
    Description:   Anti Active Gαo Mouse Monoclonal Antibody
    Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαorepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.
    Immunogen:   Recombinant full length protein of active Gαo
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                       1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG1
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Gαo antibody recognizes active Gαo of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

    Immunoprecipitation/Western blot:  
    o activation assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti active Gαo monoclonal antibody (Cat. No. 26907). Immunoblot was with an anti Gαopolyclonal antibody (Cat. No. 21015).



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