Active Gαi GTP [26901]
[30 µL]

                                             Gα_i activation assay. 
A. CHO cells were

 transfected with wild-type Gα_i1 (lanes 1 and 2) or constitutively

 active Gα_i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS 

(lane 3). Lysates were then incubated with an anti-active Gα_i monoclonal antibody 

(Cat. No.26901) (top panel). The precipitated active Gα_i was immunoblotted with 

an anti Gα_i monoclonal antibody (Cat. No. 26003). The bottom panel shows the 

Western blot with anti Gα_i monoclonal antibody (Cat.No. 26003) of the cell lysates. 

B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or 

without (lane 1)carbachol. Cell lysates were then incubated with an anti active Gα_i monoclonal antibody 

(Cat. No. 26901) (top panel).The precipitated active Ga _i was immunoblotted with an anti Gα_i rabbit polyclonal

 antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti tubulin of the cell lysates.

GI,Gai,gi