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366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | ￥2600.00 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 21271 |
| 免疫原 | Mouse |
| 规格 | 100ul |
| 技术资料 | 查看技术资料 |
BRaf [21271]
[100 µL]
| Catalog Number: 21271 |
| Gene Symbol: BRAF, BRAF1, RAFB1 |
| Description: Anti BRaf Rabbit Polyclonal Antibody |
| Background: BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| Immunogen: Purified BRaf protein, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:200 |
| Concentration: 0.1mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes BRaf of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Immunohistochemistry: |
 | Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti BRaf polyclonal antibody (Cat. #21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti mouse IgG (1:50 dilution) was used as secondary antibody. |
| Publications: |
| 1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma Mod Pathol. 2013 Mar;26(3):414-20. |
| 2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 |
| 3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma human pathology. February 2015Volume 46, Issue 2, Pages 169–175 |
| 4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 |
| 5. BRAF V600E Mutation-Specific Antibody: A Review Seminars in Diagnostic Pathology |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
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