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366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | ￥2600 |
| 品牌 | Bioyears  |
| 产地 | |
| 货号 | 21282 |
| 免疫原 | Mouse |
| 规格 | 100μl |
| 技术资料 | 查看技术资料 |
β-catenin [21282]
[100 µL]
| Catalog Number: 21282 |
| Gene Symbol: CTNNB1, CTNNB |
| Description: Anti β catenin Rabbit Polyclonal Antibody |
| Background: Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer. |
| Immunogen: A synthetic peptide from the internal region of β-catenin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes β-catenin from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 | Western blot analysis of recombinant β catenin protein. Purified His-tagged β-catenin protein (amino acids 1-76) was blotted with anti β catenin rabbit polyclonal antibody (Cat. #21282). |
| Immunohistochemistry: |
| Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-β-catenin polyclonal antibody (Cat. #21282). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody. |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
