武汉天德生物科技有限公司欢迎您！
366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 4800.00 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 26406 |
| 免疫原 | Mouse |
| 规格 | 100μl |
| 技术资料 | 查看技术资料 |
IDH1(R132P) [26406]
[100ul]
| Catalog Number: 26406 |
| Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| Description: Anti IDH1(R132P) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenases (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: A synthetic peptide from the internal region of IDH1 which includes the mutation of R132P, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: recognizes R132P mutant, but not wild-type IDH2 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 | Western blot analysis of recombinant IDH1(R132P) and wildtype proteins. Purified His-tagged IDH1(R132P) (lane 1) and corresponding wild-type IDH1 protein (lane 2) were blotted with Anti IDH1(R132P) monoclonal antibody (Cat. #26406). |
| Immunofluorescence: |
|
|
| Immunofluorescence of cells expressing IDH1 proteins with Anti IDH1(R132P) antibody. HEK293T cells were transfected with pCDNA3-GFP IDH1(R132P) plasmid, pCDNA3-GFP IDH1(WT) plasmid , pCDNA3-GFP IDH1(R132S) plasmid, pCDNA3-GFP IDH1(R132L) plasmid , pCDNA3-GFP IDH1(R132G) plasmid or pCDNA3-GFP IDH1(R132C) plasmid , then fixed and stained with Anti IDH1(R132P) monoclonal antibody (Cat. #26406). |
| Publications: |
| 1. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11 |
| 2. Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer Nature 513, 110–114 (04 September 2014) |
| 3. Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015 |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
