武汉天德生物科技有限公司欢迎您！
366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 4800.00 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 26224 |
| 免疫原 | Mouse |
| 规格 | 100μl |
| 技术资料 | 查看技术资料 |
MEK1(D67N) [26224]
[100ul]
| Catalog Number: 26224 |
| Gene Symbol: MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1 |
| Description: Anti MEK1(D67N) Mouse Monoclonal Antibody |
| Background: MEK1 (also known as extracellular signal-regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation. Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ERK1 and MAPK1/ERK2, thus activates the MAP pathway and regulates transcription. Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome (CFC) and melanoma. |
| Immunogen: A synthetic peptide from the internal region of MEK1 which includes the mutation of D67N, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes D67N mutant, but not wild-type MEK1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 | Western blot analysis of recombinant MEK1(D67N) and wildtype proteins. Purified His-tagged MEK1(D67N) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with Anti MEK1(D67N) monoclonal antibody (Cat. #26224). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing MEK1 proteins with Anti MEK1(D67N) antibody. HEK293T cells were transfected with pCDNA3-GFP MEK1(WT) plasmid (left column) or pCDNA3-GFP MEK1(D67N) plasmid (right column), then fixed and stained with Anti MEK1(D67N) monoclonal antibody (Cat. #26224). |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
