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366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 4800.00 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 26289 |
| 免疫原 | Mouse |
| 规格 | 100μl |
| 技术资料 | 查看技术资料 |
KIT(V560DEL) [26289]
[100ul]
| Catalog Number: 26289 |
| Gene Symbol: C-Kit; CD117; KIT; PBT; SCFR |
| Description: Anti KIT(V560DEL) Mouse Monoclonal Antibody |
| Background: Tyrosine-protein kinase Kit, also known as proto-oncogene c-Kit or CD117 or Mast/stem cell growth factor receptor (SCFR), is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism. |
| Immunogen: A synthetic peptide from the internal region of KIT which includes the deletion of V560, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes V560DEL mutant, but not wild-type KIT of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 | Western blot analysis of recombinant KIT(V560DEL) and wild-type proteins. Purified His-tagged KIT(V560DEL) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti KIT(V560DEL) monoclonal antibody (Cat. #26289). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing KIT proteins with Anti KIT(V560DEL) antibody. HEK293T cells were transfected with pCDNA3-GFP KIT(WT) plasmid (left column) or pCDNA3-GFP KIT(V560DEL) plasmid (right column), then fixed and stained with Anti KIT(V560DEL) monoclonal antibody (Cat. #26289). |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
