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Anti CTCF(R339W) Mouse Monoclonal Antibody

价格¥4800.00
品牌Bioyears   
产地美国
货号26326
免疫原Mouse
规格100μl
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  • 概述

    CTCF(R339W) [26326]
    [100ul]


    Catalog Number:  26326
    Gene Symbol:  CTCF; MRD21
    Description:   Anti CTCF(R339W) Mouse Monoclonal Antibody
    Background:   Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC binding factor is atranscription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation,insulator activity, recombination and regulation of chromatin architecture.
    Immunogen:   A synthetic peptide from the internal region of CTCF which includes the mutation of R339W, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes R339W mutant, but not wild-type CTCF of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles

    Western blot:  
    Western blot analysis of recombinant CTCF(R339W) and wild-type proteins. Purified His-tagged CTCF(R339W) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti CTCF(R339W) monoclonal antibody (Cat. #26326).
    Immunofluorescence:  
    Immunofluorescence of cells expressing CTCF proteins with Anti CTCF(R339W) antibody. HEK293T cells were transfected with pCDNA3-GFP CTCF(WT) plasmid (left column) or pCDNA3-GFP CTCF(R339W) plasmid (right column), then fixed and stained with Anti CTCF(R339W) monoclonal antibody (Cat. #26326).

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