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366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 4800.00 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 26199 |
| 免疫原 | Mouse |
| 规格 | 100μl |
| 技术资料 | 查看技术资料 |
BRAF(V600A) [26199]
[100UL]
| Catalog Number: 26199 |
| Gene Symbol: BRAF, BRAF1, RAFB1 |
| Description: Anti BRAF(V600A) Mouse Monoclonal Antibody |
| Background: BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| Immunogen: A synthetic peptide from the internal region of BRAF which includes the mutation of V600A, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes V600A mutant, but not wild-type BRAF of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 | Western blot analysis of recombinant BRAF proteins. Purified His-tagged BRAF(V600A) protein (lane 1) , corresponding wildtype protein (lane 2) , His-tagged BRAF(V600R) protein (lane 3), His-tagged BRAF(V600M) protein (lane 4), His-tagged BRAF(V600K) protein (lane 5), His-tagged BRAF(V600G) protein (lane 6), His-tagged BRAF(V600D) protein (lane 7) and His-tagged BRAF(V600E) protein (lane 8) were blotted with Anti BRAF(V600A) monoclonal antibody (Cat. #26199). |
| Immunofluorescence: |
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Immunofluorescence of cells expressing BRAF proteins with Anti BRAF(V600A) antibody. HEK293T cells were transfected with pCDNA3-GFP BRAF(WT) plasmid, pCDNA3-GFP BRAF(V600A) plasmid, pCDNA3-GFP BRAF(V600D) plasmid, pCDNA3-GFP BRAF(V600G) plasmid pCDNA3-GFP-BRA (V600M) plasmid or pCDNA3-GFP BRAF(V600E) plasmid, then fixed and stained with Anti BRAF(V600A) monoclonal antibody (Cat. #26199). |
| Publications: |
| 1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma Mod Pathol. 2013 Mar;26(3):414-20. |
| 2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 |
| 3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma human pathology. February 2015Volume 46, Issue 2, Pages 169–175 |
| 4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 |
| 5. BRAF V600E Mutation-Specific Antibody: A Review Seminars in Diagnostic Pathology |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
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