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Anti ERBB2(D769Y) Mouse Monoclonal Antibody

价格¥4800.00
品牌Bioyears   
产地美国
货号26382
免疫原Mouse
规格100μl
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  • 概述

    ERBB2(D769Y) [26382]
    [100ul]

    Catalog Number:  26382
    Gene Symbol:  ERBB2/HER2/Neu/c-Neu
    Description:   Anti ERBB2(D769Y) Mouse Monoclonal Antibody
    Background:   The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important critiria in clinic evaluating of the progression of breast cancer.
    Immunogen:   A synthetic peptide from the internal region of ERBB2 which includes the mutation of D769Y, human origin.
    Applications:   ELISA, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:2000 
    IF: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes D769Y mutant, but not wild-type ERBB2 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles

    Immunofluorescence:  
    Immunofluorescence of cells expressing ERBB2 proteins with Anti ERBB2(D769Y) antibody. HEK293T cells were transfected with pCDNA3-GFP ERBB2(D769Y) plasmid, pCDNA3-GFP ERBB2(WT) plasmid or pCDNA3-GFP ERBB2(D769H) plasmid, then fixed and stained with Anti ERBB2(D769Y) monoclonal antibody (Cat. #26382).


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