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Anti GNAQ(Q209L) Mouse Monoclonal Antibody

价格4800
品牌Bioyears   
产地美国
货号26328
免疫原Mouse
规格100ul
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  • 概述

    GNAQ(Q209L) 26328
    [100ul]


    Catalog Number:  26328
    Gene Symbol:  GNAQ, CMC1, G-ALPHA-q, GAQ, SWS
    Description:   Anti GNAQ(Q209L) Mouse Monoclonal Antibody
    Background:   GNAQ is a protein that in humans encoded by the GNAQ gene. The GNAQ protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospholipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation.
    Immunogen:   A synthetic peptide from the internal region of GNAQ which includes the mutation of Q209L, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes Q209L mutant, but not wild-type GNAQ of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles

    Western blot:  
    Western blot analysis of recombinant GNAQ(Q209L) and wildtype proteins. Purified His-tagged GNAQ(Q209L) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti GNAQ(Q209L) monoclonal antibody (Cat. #26328).
    Immunofluorescence:  
    Immunofluorescence of cells expressing GNAQ proteins with anti GNAQ(Q209L) antibody. HEK293T cells were transfected with pCDNA3-GFP GNAQ(Q209L) plasmid pCDNA3-GFP GNAQ(WT) plasmid, pCDNA3-GFP GNAQ(Q209P) plasmid or pCDNA3-GFP GNAQ(Q209R) plasmid, then fixed and stained with anti GNAQ(Q209L) monoclonal antibody (Cat. #26328).



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