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366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 4800 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 26272 |
| 免疫原 | Mouse |
| 规格 | 100ul |
| 技术资料 | 查看技术资料 |
FBXW7(R479L) [26347]
[100ul]
| Catalog Number: 26347 |
| Gene Symbol: FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10 |
| Description: Anti FBXW7(R479L) Mouse Monoclonal Antibody |
| Background: F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. |
| Immunogen: A synthetic peptide from the internal region of FBXW7 which includes the mutation of R479L, human origin. |
| Applications: ELISA, WB |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes R479L mutant, but not wild-type FBXW7 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 | Western blot analysis of recombinant FBXW7(R479L) and wildtype proteins. Purified His-tagged FBXW7(R479L) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti FBXW7(R479L) monoclonal antibody (Cat. #26347). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing FBXW7 proteins with Anti FBXW7(R479L) antibody. HEK293T cells were transfected with pCDNA3-GFP FBXW7(WT) plasmid (left column) or pCDNA3-GFP FBXW7(R479L) plasmid (right column), then fixed and stained with Anti FBXW7(R479L) monoclonal antibody (Cat. #26347). |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
