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366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 4800 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 26160 |
| 免疫原 | Mouse |
| 规格 | 100ul |
| 技术资料 | 查看技术资料 |
IDH1(R132S) [26160]
[100 µL]
| Catalog Number: 26160 |
| Gene Symbol: IDH1; IDCD; IDH; IDP; IDPC; PICD |
| Description: Anti IDH1(R132S) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: A synthetic peptide from the internal region of IDH1 which includes the mutation of R132S, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes IDH1(R132S) mutated prteein, but not wild-type IDH1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 | Western blot analysis of recombinant IDH1(R132S) and wild-type proteins. Purified His-tagged IDH1(R132S) protein (lane 2) and wild-type protein (lane 1) were blotted with Anti IDH1(R132S) mouse antibody (Cat. #26160). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing IDH1 proteins with Anti IDH1(R132S) antibody. HEK293T cells were transfected with pCDNA3-GFP IDH1(WT) plasmid (left column) or pCDNA3-GFP IDH1(R132S) plasmid (right column), then fixed and stained with Anti IDH1(R132S) monoclonal antibody (Cat. #26160). |
| Publications: |
| 1. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11 |
| 2. Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer Nature 513, 110–114 (04 September 2014) |
| 3. Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015 |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
