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366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 4800 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 26084 |
| 免疫原 | Mouse |
| 规格 | 100ul |
| 技术资料 | 查看技术资料 |
IDH2(R172H) [26084]
[100 µL]
| Catalog Number: 26084 |
| Gene Symbol: IDH2 |
| Description: Anti IDH2(R172H) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-172 in IDH2 to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: A synthetic peptide from the internal region of human IDH2 which includes the mutation of R172H. |
| Applications: ELISA, WB, IHC, IF |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 IF: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-IDH2(R172H) antibody recognizes IDH2(R172H) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
| Western blot: |
 | Western blot analysis of recombinant IDH2(R172H) and wild-type IDH2 proteins. Purified his-tagged IDH2(R172H) protein (Cat. # 10201) and wild-type IDH2 protein (Cat. # 10200) were blotted with anti IDH2(R172H) monoclonal antibody (Cat. # 26084) (left panel). Proteins loading were blotted with anti-IDH2 monoclonal antibody (right panel). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing IDH2 proteins with Anti IDH2(R172H) antibody. HEK293T cells were transfected with pCDNA3-GFP IDH2(WT) plasmid (left column) or pCDNA3-GFP IDH2(R172H) plasmid (right column), then fixed and stained with Anti IDH2(R172H) monoclonal antibody (Cat. #26084). |
| Publications: |
| 1. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11 |
| 2. Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer Nature 513, 110–114 (04 September 2014) |
| 3. Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015 Fresia Pareja, Edaise M. da Silva, [..] Edi Brogi E 02 January 2020 |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
