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366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 4800 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 26082 |
| 免疫原 | Mouse |
| 规格 | 100ul |
| 技术资料 | 查看技术资料 |
EGFR(L858R) [26082]
[100 µL]
| Catalog Number: 26082 |
| Gene Symbol: EGFR/HER1/ERBB1 |
| Description: Anti EGFR(L858R) Mouse Monoclonal Antibody |
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS RAF MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). |
| Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of L858R, human origin. |
| Applications: ELISA,WB,IF,IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG1 |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes EGFR(L858R), but not wild type EGFR from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
| Western blot: |
 | Western blot analysis of recombinant EGFR(L858R) and wildtype proteins. Purified His-tagged EGFR(L858R) protein (amino acid 702-987, lane 2, Cat. # 10230) and corresponding wild type protein (amino acid 702-987, lane 1, Cat. # 10210) were blotted with anti EGFR(L858R) mouse monoclonal antibody (Cat. # 26082) . Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. # 28004). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing EGFR proteins with anti EGFR(L858R) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP EGFR(L858R) plasmid (right column), then fixed and stained with anti- EGFR(L858R) monoclonal antibody (Cat. # 26082). |
| Immunohistochemistry: |
| Immunohistochemical analysis of paraffin-embedded human NSCLC tissue with anti-EGFR(L858R) monoclonal antibody (Cat. #26082). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:400) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:100) was used as secondary antibody. |
| Publications: |
| 1. Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma Tumor Biology. October 2014 |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
