武汉天德生物科技有限公司欢迎您！
366577646@qq.com
366577646@qq.com
| 027-87561633
027-87561633366577646@qq.com
| 价格 | 4800 |
| 品牌 | Bioyears  |
| 产地 | 美国 |
| 货号 | 21202 |
| 免疫原 | Mouse |
| 规格 | 100ul |
| 技术资料 | 查看技术资料 |
ERBB2 [21202]
[100 µL]
| Catalog Number: 21202 |
| Gene Symbol: ERBB2/HER2/Neu |
| Description: Anti ErbB2 Rabbit Polyclonal Antibody |
| Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. |
| Immunogen: A synthetic peptide from the central of ErbB2 protein. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:500-1:5000 WB: 1:200-1:2000 IHC: 1:20-1:200 |
| Concentration: 0.2 mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes ErbB2 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
| Western blot: |
 | Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (Cat. #10208) was blotted with Anti ErbB2 rabbit polyclonal antibody (Cat. #21202). |
| Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Anti ErbB2 polyclonal antibody (Cat. #21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody. |
电话： 027-87561633
邮箱： Bioyears@126.com
电话： 027-87561633
邮箱： Bioyears@126.com
地址： 武汉市东湖开发区关东科技工业园3#产业区3-3栋15号
