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Anti active Arl1 Mouse Monoclonal Antibody

价格4800
品牌Bioyears   
产地美国
货号26924
免疫原Mouse
规格30μl
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  • 概述

    Active Arl1 GTP [26924]
    [30 µL]


    Catalog Number:  26924
    Gene Symbol:  Arl1
    Description:   Anti active Arl1 Mouse Monoclonal Antibody
    Background:   Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal.
    Immunogen:   Recombinant Arl1 protein (active form)
    Applications:   IP, IHC, IF
    Recommended Dilutions:   
    1 µg for 1~2 mg total cellular proteins for IP 
    1:50~100 dilution for IHC, IF
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Arl1 antibody recognizes active Arl1-GTP from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

    Immunoprecipitation/Western blot:  
    Arl1 Activation Assay. Purified GST-tagged Arl1 proteins (Cat. #10136) were immunoprecipitated with the anti active Arl1 monoclonal antibody (Cat. # 26924) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti Arl1 monoclonal antibody (Cat. #26056). Arl1 protein input control is shown in the bottom panel.



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